Immunocytochemical analysis of EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using human EOMES Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47818) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper pane and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.